Composite

Part:BBa_K4147007:Design

Designed by: Ana Belem García González   Group: iGEM22_Tec-Chihuahua   (2022-10-08)


PcOSM Construct with DsbA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 806
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1184
    Illegal NgoMIV site found at 1368
    Illegal AgeI site found at 498
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When adding a protein for co-expression, like in this case, make sure to put between the CDS and the signal peptide an RBS that allows independent transcription of each protein.


Source

GenBank: EU271754.1 https://www.ncbi.nlm.nih.gov/nuccore/EU271754.1


References